FastQCFastQC Report
Mon 25 Sep 2023
Tp13_S13_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTp13_S13_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12543741
Sequences flagged as poor quality0
Sequence length64-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGCCGCGAAAATTCGTTTTTATTATTCAACATAGTTCCCTTCAAGAGCGATACAACGATTATAACGACCTTCC295690.23572712478677613No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAG207040.16505442833999842No Hit
CAATCATTAATGGTACAAGAGTGATGAAAATACCGTAGCCCATCCATTCAAGACCGGAACCATTGGTGAGTGTGT156560.12481125048739447No Hit
GCGGCCGCATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACT132550.10567023027659771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)